Relationship between chromatin bridges in anaphase and chromosomal aberrations induced by TMP+UVA (365 nm) in CHO cells

Abstract

In a recent paper, the hypothesis of `conservative pairing' between complementary DNA strands belonging to both sister chromatids has been proposed as a phenomenon that could account for, at least in part, sister chromatid pairing in late G 2/mitosis. The hypothesis was verified through a cytogenetic approach, studying the so-called `sister chromatid chromatin bridges' (SCCBs), induced in the previous G 2/mitosis by a crosslinking (TMP+UVA 365 nm) treatment in CHO cells (Rizzoni, M., E. Cundari, P. Perticone and B. Gustavino (1993) Chromatin bridges between sister chromatids induced in late G 2 mitosis in CHO cells by trimethylpsoralen+UVA, Experimental Cell Res., 209, 149–155; [1]). The purpose of the present paper is the study of the relationship between chromatin bridges without fragments in ana–telophase, which were demonstrated to be SCCBs, and chromosomal aberrations, in order to investigate their mechanism of induction. The evolution along the time of the two classes of mitotic anomalies was studied and a comparison was carried out to verify whether the bridges rise as a direct and immediate effect of the treatment or represent the misrepair-mediated effect of it. The present data show that single bridges without fragments come from a direct effect of photoinduced crosslinks in late G 2/mitosis. Moreover TMP+365 nm UVA treatment shows an S-dependent clastogenic effect. The proposed hypothesis of `conservative pairing' between DNA strands of sister chromatids is further supported.