Abstract
The deconjugation rate in vitro of bilirubin and phenolphthalein glucuronides by β-glucuronidase of liver homogenates and blood sera from normal and bile ductligated rats was studied. Distribution of bilirubin and β-glucuronidase activity in serum, liver and skin was also investigated. Rates of deconjugation of phenolphthalein glucuronide in vitro showed a positive and significant correlation with those of conjugated bilirubin. Deconjugation for both substrates was increased by serum and liver from ligated rats as compared to controls. β-Glucuronidase activity increased in liver 24 h after ligature and was released to the blood stream where a maximum value was observed on the fifth day after ligature. Determination of bilirubin in skin revealed a progressive increase of indirect reacting bilirubin in accordance with days of biliary obstruction. Serum β-glucuronidase activity could be directly related to indirect reacting bilirubin present in skin.
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