Abstract
The terpenoid phytoalexins hemigossypol (HG), methoxyhemigossypol (MHG), desoxyhemigossypol (dHG), and desoxymethoxyhemigossypol (dMHG) from Verticillium dahliae-infected, wilt-resistant Seabrook Sea Island cotton were tested at pH 6.3–7.5 in liquid nutrient media for toxicity to two defoliating and two nondefoliating isolates of V. dahliae. Terpenoid concentrations of 20, 40, 50, and 90 μg/ml of dHG, HG, dMHG, and MHG, respectively, were required to kill uniformly mycelia of all four isolates of V. dahliae. Using this bioassay, differential sensitivity to terpenoid phytoalexins does not appear to be a factor in the differences in virulence of the defoliating and nondefoliating isolates of V. dahliae on cotton.
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