Abstract
We describe a new abdominal rehydration technique based on ammonium hydroxide and sodium bicarbonate. We present the variations resulting from different rehydration times. Also, we compared six aspects of this technique and those based on proteinase K, potassium hydroxide, and distilled water. This rehydration technique can be of great value since it shows similar results to those recorded in fresh material. These surpass those obtained with other previously studied methods. The refinement of these procedures with the addition of further proposals —from the forensic sciences— could improve the recovery of the chorion in females of Lepidoptera from collections. The last would allow choosing a technique according to the advantages and disadvantages, depending on the specimens' condition: size, volume, and type of egg.
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