Regulation of proline accumulation in Arabidopsis thaliana (L.) Heynh during development and in response to desiccation

Abstract

ABSTRACTSignificant differences were observed in the amount and proportion of free amino acids in different organs of Arabidopsis thaliana (L.) Heynh, ecotype Columbia. The most notable were found for proline, which formed 17–26% of the total free amino acid concentration in reproductive tissues (floret and seed), but only 1–3% of the total free amino acid concentration in vegetative tissues (rosette leaf and root). Proline accumulation was associated with tissues that had relatively low water contents. Tissues which displayed high water contents, such as rosette leaves, contained low levels of proline. A significant increase in the levels of proline accumulation occurred in plants subjected to experimentally induced low water potentials as compared to unstressed plants. For instance, an 8–10‐fold increase in proline was observed in the presence of 120 mmol kg−1 NaCl or KCl, and a 20‐fold increase was stimulated by 60 mmol kg−1 PEG. However, in addition to the accumulation of proline, massive accumulation of Na+, K+ and Cl− ions occurred in tissues of plants stressed with salt. No significant differences were observed in mineral ions in plants stressed with PEG. Isotope tracer experiments with 14C compounds established that glutamate, ornithine and arginine are precursors of the proline biosynthesis induced by PEG in response to low water potentials in Arabidopsis thaliana. We conclude that the accumulation of proline in response to PEG occurs through increased biosynthesis.