Abstract
Summary: Immunoelectrophoresis, using antibodies raised against homogeneously pure nitrate reductase (NR; EC 1.6.6.2), was used to study the regulation of NR synthesis in Candida nitratophila. Extracts from ammonium-grown cultures did not contain cross-reacting material, but cross-reacting material was detected in cultures transferred to nitrogen-free and nitrate medium. The appearance of NR activity in these extracts appeared to be the result of de novo protein synthesis. The possible role of nitrate in the stimulation of active NR synthesis is discussed. Losses of NR activity that occurred in nitrate-grown cultures transferred to ammonium medium correlated with decreases in cross-reacting material.
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