Abstract
The zinc cluster proteins Sut1 and Sut2 play a role in sterol uptake and filamentous growth in the budding yeast Saccharomyces cerevisiae. In this study, we show that they are also involved in mating. Cells that lack both SUT1 and SUT2 were defective in mating. The expression of the genes NCE102 and PRR2 was increased in the sut1 sut2 double deletion mutant suggesting that Sut1 and Sut2 both repress the expression of NCE102 and PRR2. Consistent with these data, overexpression of either SUT1 or SUT2 led to lower expression of NCE102 and PRR2. Furthermore, expression levels of NCE102, PRR2 and RHO5, another target gene of Sut1 and Sut2, decreased in response to pheromone. Prr2 has been identified as a mating inhibitor before. Here we show that overexpression of NCE102 and RHO5 also reduced mating. Our results suggest that Sut1 and Sut2 positively regulate mating by repressing the expression of the mating inhibitors NCE102, PRR2 and RHO5 in response to pheromone.
Highlights
Sut1 and Sut2 of the budding yeast Saccharomyces cerevisiae are transcriptional regulators of the Zn(II)2Cys6 family, which is known as zinc cluster family [1,2]
It has previously been shown that the transcriptional regulators Sut1 and Sut2 play a role in sterol import and filamentation [2,4,5,6]
We demonstrate that Sut1 and Sut2 regulate mating
Summary
Sut and Sut of the budding yeast Saccharomyces cerevisiae are transcriptional regulators of the Zn(II)2Cys family, which is known as zinc cluster family [1,2]. The two corresponding genes are paralogs that were formed by whole genome duplication [3]. Both proteins are involved in sterol import and filamentous growth but molecular mechanisms have only been characterized for Sut1 [2,4,5,6]. Overexpression of either SUT1 or SUT2 leads to inhibition of filamentous growth suggesting that the corresponding proteins are negative regulators of filamentation [5,6]. Sut binds to the promoter regions of GAT2, HAP4, MGA1, MSN4, NCE102, PRR2, RHO3, and RHO5, and increased SUT1 levels result in lower expression of these genes [6,11].
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