Abstract
To precisely analyze the role of ovarian steroids in the regulation of laminin chain gene expression in mouse uterine tissues, the ovariectomized mouse model was used. Ovariectomized mice received a single injection of steroid hormones and total RNA was isolated from whole uterine tissues. Messenger RNA levels of each laminin chain (A, B1, and B2) were determined by competitive RT‐PCR procedures. Estradiol decreased mRNA levels of laminin B1 chain about two‐fold, and B2 chain rather moderately. Estradiol‐induced inhibition of laminin B1 and B2 chain mRNA levels were completely blocked by pretreatment with estrogen receptor antagonist tamoxifen. Estriol, a short acting estrogen which cannot induce hyperplastic responses of rodent uterine tissues, also showed an inhibitory effect on B1 and B2 chain mRNA levels, while estrone, an inactive estrogen, failed to influence either B chain mRNA levels. Effects of steroids on A chain mRNA level were quite different from those on B chains. Laminin A chain mRNA level ...
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