Abstract
Acidovorax facilis 72W nitrilase catalyzed the regioselective hydrolysis of ( E, Z)-2-methyl-2-butenenitrile, producing only ( E)-2-methyl-2-butenoic acid with no detectable conversion of ( Z)-2-methyl-2-butenenitrile. ( E)-2-Methyl-2-butenoic acid, produced in aqueous solution as the ammonium salt, was readily separated from ( Z)-2-methyl-2-butenenitrile, and isolated in high yield and purity. The combination of nitrile hydratase and amidase activities of several Comamonas testosteroni strains were also highly regioselective for the production of ( E)-2-methyl-2-butenoic acid from ( E, Z)-2-methyl-2-butenenitrile.
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