Regional differences in the morphology of the goat epididymis: A light microscopic and ultrastructural study

Abstract

The goat epididymis, based on morphological differences, was divided into five regions; regions I and II, and the proximal part of region III constituted the head; the distal part of region III and region IV, the body; and region V, the tail. The epithelium of all regions contained principal and basal epithelial cells and intraepithelial lymphocytes and macrophages. In addition, regions II to IV also contained a few apical cells. Clear cells were absent. The epithelium varied in height from the tallest in region I (88 +/- 33 microns) to the shortest in region V (38 +/- 5 microns). Conversely, the luminal diameter, thickness of smooth muscle wall, and luminal sperm concentration were highest in region V. The irregular epithelial height of regions I and IV accounted for a stellate lumen in contrast to the oval lumen of the other regions. Whereas the lumen of region I contained only a few sperm, those of regions II, III, and IV were filled with sperm. Principal cells were the only cell type that showed striking cytological differences between regions. While they contained absorptive features (canaliculi, pinocytotic and coated vesicles, and subapical vacuoles) in all regions, the principal cells of region II were filled with large, heterogeneous vacuoles (up to 5 microns in diameter), suggesting that they may be preferentially involved in transporting and digesting particulate material. Besides absorptive features, principal cells of all regions contained morphological correlates of protein synthesis such as highly developed Golgi complexes in the supranuclear area and numerous cisternae of RER near the Golgi body and in the infranuclear cytoplasm. The cisternae of RER were more developed in region IV, and in some instances, they were distended with flocculent material resembling newly synthesized protein. Unlike the protein synthesizing organelles, principal cells of all regions lacked morphological correlates of steroid hormone synthesis. These results are compared with previously published data on the regional differences in the epididymis of other species, especially with those of the rat and the bull, in an effort to understand the significance of the epididymis in sperm maturation.