Abstract
An acetonitrile-utilising bacterium AJ270 has been isolated from soil, identified as a Rhodococcus sp. and shown to be distinct from all the recognised species of the genus. It grows well on 32 of 36 aliphatic, aromatic and hetero-aromatic nitriles tested and is capable of rapid growth on high concentrations (0.25–0.38 M) of acetonitrile, benzonitrile and 3-cyanopyridine. The nitrile hydratase of Rhodococcus AJ270 is stable on storage for 18 months at − 20° C, has activity against a very broad range of nitriles and dinitriles and is able to catalyse regio-specific and stereo-specific nitrile biotransformations. The suitability of AJ270 as a robust and versatile biocatalyst is discussed.
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