Abstract
Aim of investigation . To prove the usefulness of the use of embryonic fibroblasts for the regeneration of cartilaginous tissue. Material and methods . New experimental model of symphysis pubis rupture followed by its recovery was employed in 24 male Shinshilla rabbits (2.3—3.5 kg body mass) in 3 series of experiments. In the first series, reconstruction was not employed. In the 2nd series, the reconstruction of symphisis was performed with the aid of tantal wired cerclage (d=1.2 mm). In the 3d series, the reconstitution was the same as in the previous series of experiments with addition of grown embrional allofibroblast tissue pieces. Duration of experiments was 7, 14, 21and 30 days. After each experiment, pieces of cartilaginous tissue were fixed with 10% neutral formalin and embedded in paraffin. Histological sections were stained with hematoxylin, eosin and VanGieson. Results. After the rupture of symphysis pubis of the pelvic bone, the cartilaginous tissue undergone alterations followed by formation of chondromatous nodules of different sizes and sclerosis of surrounding muscular and osseous tissues. In the second series of experiment after pelvis symphysis pubis rupture and symphysis recovery with the help of cerclage, the morphologic investigation revealed the formation of mixed initial callus consisting of connective and chondromatous tissue elements. In the third series of experiment it has been shown that within 4 weeks of experiment, the connective tissue with intensive basophil stain is formed in the area of symphysis pubisrupture. Connective tissue formation in surrounding soft tissue structures was associated with the formation of cartilaginous cells. Conclusion. Process of formation of cartilaginous tissue in the area of symphysis pubis rupture as evident by the experimental observation demonstrates the stimulating influence of allogeneic fibroblasts on process of cartilaginous tissue regeneration that ensures premises for clinical development of suggested method of regeneration.
Highlights
Регенерация — важнейший механизм вос становления структуры и функции органов при критических состояниях [1, 2]
In the first series of experiments, a tentative model of pubic joint rupture has been developed as a control and the process of cartilaginous tissue regeneration in the dynamics has been evaluated during four weeks
Morphological investigations performed after developing the pubic joint rupture showed that at early period (1st week) of experiment the cartilage defect was filling up with necrotic mass and blood elements, the circumflex cartilaginous tissue underwent disorganiza tion, dystrophic and destructive alterations
Summary
Регенерация — важнейший механизм вос становления структуры и функции органов при критических состояниях [1, 2]. В последние го ды в качестве способа стимуляции процесса ре парации тканей предлагается использовать культивированные аллофибробласты [3—8]. Не которые авторы сообщают о положительных клинических результатах их применения [4—6, 8—11]. Большое количество предложенных ме тодов остеосинтеза свидетельствует о необходи мости продолжения поиска более совершенных, доступных, экономически менее затратных спо собов стимуляции репаративного остеогенеза, которые позволили бы сохранить преимущества традиционных методов и максимально сокра тить их недостатки [13—19]. Одним из возмож ных способов стимуляция репаративного остео и хондрогенеза является применение эмбрио нальных аллофибробластов [7, 8, 10—12]. Цель исследования — обоснование примене ния эмбриональных аллофибробластов в процес се регенерации хрящевой ткани
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