Abstract
In order to develop an adventitious regeneration system for Colt cherry root segments several experiments were conducted to study the role of important parameters of culture conditions, regeneration media and explant sources. One centimeter long root segments of in vitro produced roots were used as explants on Murashige and Skoog (MS') basic medium supplemented with either thidiazuron (TDZ) or benzylaminopurine (BAP) at different concentrations. The two different cytokinins promoted the formation of completely different adventitious structures: BAP at all effective concentrations induced somatic embryo production through a callus phase at the cut of root segments, whereas in the presence of TDZ only adventitious shoots originated directly from the root perycicle. Optimal concentrations for BAP and TDZ were 6.6 M and 15 M respectively. A dark incubation period of two weeks prior to exposing the cultures to a 16 h photoperiod affected BAP and TDZ effect in different ways, that of BAP being obviously enhanced and that of TDZ almost unaffected or slightly inhibited.
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