Abstract
A method for growing plant protoplasts immobilized in Ca-alginate is presented in detail. Two types of protoplasts, isolated from hypocotyls of Brassica napus cv. Niklas and leaf mesophyll tissue of Nicotiana sanderae cv. Crimson Bedder, have been used as examples to illustrate the general applicability of the method. The major advantage of the method is improved cellular protection during the first critical weeks after isolation. This is a protection both against mechanical strain and strong gradients in environmental conditions. Higher plating efficiency may thus be achieved and handling simplified, particularly when changing nutrient medium. Both regeneration and differentiation have repeatedly been achieved.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
