Regenerasi dan Pertumbuhan Beberapa Varietas Tebu (Saccharum officinarum L.) secara In Vitro

Abstract

<p>(Saccharum officinarum L.) through In Vitro Culture.<br />Deden Sukmadjaja and Ade Mulyana. The research was<br />conducted at the Laboratory of Tissue Culture The Biology of<br />Cell and Tissue Researcher Group ICABIOGRAD, Bogor from<br />June to November 2009 to studied growth and regenerations<br />response some varieties of sugarcane through in vitro<br />culture. The research activities have been carried out in<br />three steps, i.e., callus formation, regeneration of shoots and<br />roots regeneration. The type of explants used in the study<br />was in vitro planlet explants of both sugarcane varieties.<br />Seven media formulations were used for the callus induction<br />and regeneration of shoots, while five media formulations<br />were used for the roots regeneration. The results<br />showed that the highest respond for calluses induction was<br />Bulu Lawang varieties at media formulation MS + 2.4-D 2<br />mg.l-1 + BAP 0.4 mg.l-1 + CH 2000 mg.l-1 and PS 951 varieties<br />at media formulation MS + 2.4-D 1 mg.l-1 + BAP 0.4 mg.l-1.<br />While the highest respond for regeneration of shoots was<br />Bulu Lawang varieties at media formulation MS0 (control<br />MS) dan PS 951 varieties at media formulation MS + BAP 1<br />mg.l-1 + kinetin 1 mg.l-1 + NAA 0.5 mg.l-1 + GA3 0.5 mg.l-1.<br />The highest respond of roots regeneration was Bulu Lawang<br />and PS 951 varieties at media formulation MS + IBA 1 mg.l-1.<br />Acclimatization of plantlets produced were grew successfully<br />about 90-100% in greenhouse.</p>