Abstract
In 2018, a 26-month-old girl, fully vaccinated with Rotarix in 2016, presented with fever, diarrhea, and vomiting. A rapid test showed that her feces contained rotavirus A (RVA). VP7 reverse transcription-PCR (RT-PCR) and Illumina sequencing showed that a G1P[8] strain with a Wa-like genotype constellation was the etiologic agent. This is the first near-complete RVA genome sequence from Nigeria.
Highlights
In 2018, a 26-month-old girl, fully vaccinated with Rotarix in 2016, presented with fever, diarrhea, and vomiting
The sample was subjected to a rapid immunochromatographic test for both rotavirus A (RVA) and enteric adenoviruses
Afterwards, the sample was subjected to the novel enrichment technique of the VIRomes (NetoVIR) protocol (5)
Summary
In 2018, a 26-month-old girl, fully vaccinated with Rotarix in 2016, presented with fever, diarrhea, and vomiting. A fecal sample was collected in 2018 from a 26-month-old girl who presented with fever, diarrhea, and vomiting. The sample was subjected to a rapid immunochromatographic test for both RVA and enteric adenoviruses (rotavirus group A antigen/enteric adenovirus antigen rapid test kit [AccuMed Technology Co., Ltd., Beijing, China]). RNA was extracted, followed by cDNA synthesis, VP7 nested multiplex PCR, and gel electrophoresis (4).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
