Abstract
BackgroundDengue virus infection has recently taken endemic proportion in India implicating all the four known dengue serotypes. There was a major dengue outbreak in northern India including Delhi in October- December, 2003 and again in 2004. We have carried out a detailed investigation of the 2004 outbreak by Serosurveillance, RT-PCR, nested PCR, virus isolation and genotyping. We also report the molecular epidemiological investigation of these outbreaks.ResultsThe serological investigation of 162 suspected serum samples using an in-house dengue dipstick ELISA revealed 11%-IgM, 51%-IgG and 38%-both IgM and IgG antibody positivity. The RT-PCR analysis revealed presence of dengue RNA in 17 samples. Further subtyping and genotyping by nested PCR and nucleotide sequencing of C-prM gene junction revealed the association of subtype III of dengue virus type 3 in the outbreak.ConclusionThe sudden shifting and dominance of the dengue virus serotype-3 (subtype III) replacing the earlier circulating serotype-2 (subtype IV) is a point of major concern and may be attributed to increased incidence of DHF and DSS in India.
Highlights
Dengue virus infection has recently taken endemic proportion in India implicating all the four known dengue serotypes
In few cases DF leads to severe life threatening dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS)
Like antibody dependent enhancement (ADE) in heterotypic secondary dengue infections, involvement of a virulent viral genotype, and host factors have been suggested to explain the mechanism of pathogenesis of DHF and DSS [3]
Summary
Dengue virus infection has recently taken endemic proportion in India implicating all the four known dengue serotypes. We have carried out a detailed investigation of the 2004 outbreak by Serosurveillance, RT-PCR, nested PCR, virus isolation and genotyping. All the four serotypes of dengue viruses are primarily transmitted by Aedes aegypti .Infection with any one of these serotypes generally leads to a mild, self limiting febrile illness In few cases DF leads to severe life threatening dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Like antibody dependent enhancement (ADE) in heterotypic secondary dengue infections, involvement of a virulent viral genotype, and host factors have been suggested to explain the mechanism of pathogenesis of DHF and DSS [3]
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