Abstract
The aim of this study was investigate the anti-obesity effect and mechanism of action of chokeberry (Aronia melanocarpa) extract (CBE) in 3T3-L1 cells. CBE was tested for its antioxidant compounds content (total phenol and flavonoid). Lipid accumulation levels were measured by determining the expression of genes associated with adipocyte differentiation and adipokine secretion in 3T3-L1 cells. In CBE, the total phenolic content was 212.0 ± 3.11 mg/g and the flavonoids content was 113.25 ± 2.5 mg/g. Oil Red O staining showed that CBE inhibited lipid accumulation at concentration of 0, 0.5, and 1.0 mg/mL in a dose-dependent manner. CBE decreased the expression of the key adipocyte differentiation regulator peroxisome proliferator-activated receptor-γ and the fatty acid binding protein 4 gene during the differentiation of preadipocytes into adipocytes. Moreover, CBE down regulated adipokine-specific genes such as leptin and monocyte chemoattractant protein-1 relative to non-treated adipocytes. These results demonstrated that CBE inhibited adipogenesis by down regulating peroxisome proliferator-activated receptor-γ and fatty acid binding protein 4 and by reducing adipokine expression in 3T3-L1 cells.
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