Abstract
Remirea maritima is a tropical plant with a reticulated root system belonging to the family Cyperaceae, also known to have biologically active secondary metabolites. However, very few data on R. maritima’s biological actions are available and there are no reports regarding the redox-active profile of this plant. In this study, we examined the total phenolic content of Remirea maritima hydroalcoholic (RMHA) extracts, redox properties against different reactive species generated in vitro and their cytotoxic effect against fibroblasts (L929) and melanoma (B16F10) cells. Total reactive antioxidant potential index (TRAP) and total antioxidant reactivity (TAR) results revealed that RMHA at all concentrations tested showed significant antioxidant capacity. RMHA was also effective against hydroxyl radical formation, reduction of Fe3+ to Fe2+ and in scavenging nitric oxide (NO) radicals. In vitro, the level of lipid peroxidation was reduced by RMHA extract and the data showed significant oxidative damage protection. The RMHA cytotoxicity was evaluated by a neutral red assay in fibroblast (L929) and melanome (B16F10) cells. The obtained results showed that the RMHA (40 and 80 µg/mL, respectively) reduced 70% of the viable cells. In conclusion, this study represents the first report regarding the antioxidant and anti-proliferative potential of R. maritima against B16F10 melanoma cells.
Highlights
The pathogenesis of numerous chronic diseases has been related with unbalanced levels of free radicals in systemic tissues [1]
The selective index (SI) for Remirea maritima hydroalcoholic (RMHA) between fibroblast and melanoma cells was 0.65, the results showed that, it presented a significantly reduction of viable cells of B1610 melanoma cells at concentrations of 80 to 320 μg/mL, RMHA was not selective for melanoma skin cancer cells when compared with normal fibroblast cells (Figure 7)
Isovitexin is already known to exhibit antioxidant potential, to reduce the amount of hydrogen peroxide and to inhibit the production and, or release of tumor necrosis factor and prostaglandin E2 (PG2) in inflammatory processes induced by lipopolysaccharide (LPS) in mouse macrophages. These findings suggested that suppression of reactive oxygen species (ROS)-mediated COX-2 expression by isovitexin is beneficial in reducing inflammation and carcinogenesis [53]
Summary
The pathogenesis of numerous chronic diseases has been related with unbalanced levels of free radicals in systemic tissues [1]. Epidemiological surveys indicate that the diet plays an important role in preventing chronic diseases due to the antioxidants it supplies [2,3,4]. Plant-based foods contain significant amounts of phytochemicals which possess numerous health benefits [5]. These phytochemicals are mostly phenols, which can be divided into at least 10 types depending on their basic structure. Several studies have previously reported that many of these phytochemicals have antioxidant activity, and anticarcinogenic and antimutagenic effects by inhibiting cell proliferation [7,8,9,10]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
