Abstract
Reactor configuration was investigated for optimal production of red pigment by Carthamus tinctorius cells in a two-stage culture. For the first stage, growth levels of cells in an Erlenmeyer flask on a rotary shaker and a seesaw type reactor were higher than those in an STR and bubble column. Cells grown at high k L a conditions possessed high pigment forming capacity in the second culture stage. Product inhibition was observed for pigment formation in the second culture stage. High pigment production levels were achieved by periodical removal of pigment from the reactor. Production medium in the second culture stage could be reused by removal of the pigment formed.
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