Abstract

Exhausted olive pomace (EOP) is a residue derived from the olive pomace oil industry. One of the main components of this agro-industrial residue is the extractive fraction which contains non-structural components such as bioactive compounds. In this work, different extraction methods, including green technologies, have been compared to evaluate the extraction of antioxidants from EOP: hydrothermal extraction, aqueous accelerated extraction, organosolv extraction, and extraction with aqueous salt solutions. The extracts obtained were characterized regarding the content of total phenols by the Folin–Ciocalteu method. After characterization, hydroxytyrosol was found to be one of the potential active compounds in EOP.

Highlights

  • Fruit olive tree and olive oil are two of the main food products in the Mediterranean basin [1,2]

  • Gallic acid was used as a reference standard compound and the results were expressed as mg of gallic acid equivalents (GAE)/g exhausted olive pomace (EOP)

  • The valorization of this agro-industrial residue implies the utilization of this fraction, which contains bioactive compounds

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Summary

Introduction

Fruit olive tree and olive oil are two of the main food products in the Mediterranean basin [1,2]. EOP is the residual solid biomass obtained after the extraction of the olive pomace oil, generally, with hexane It presents a high content of nonstructural components, around 41% [4], and for this reason, it constitutes a promising feedstock bioactive compounds extraction, such as phenolic compounds [5]. Since EOP has been little explored, the aim of this work was to obtain antioxidants from EOP, using different extraction methods, including green technologies Both conventional techniques such as hydrothermal extraction, organosolv extraction and aqueous extraction using saline conditions and a new extraction technique, accelerated solvent extraction, were applied. The phenolic extract profiles were identified by high performance liquid chromatography (HPLC)-UV

Chemical and Standards
Conditions Applied in the Extraction Methods
Total Phenolic Content
Phenolic Profile by HPLC
Evaluation of the Extraction Conditions
Phenolic Profiles
Conclusions
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