Abstract
The mitis group of streptococci (MGS) is a member of the healthy human microbiome in the oral cavity and upper respiratory tract. Troublingly, some MGS are able to escape this niche and cause infective endocarditis, a severe and devastating disease. Genome-scale models have been shown to be valuable in investigating metabolism of bacteria. Here we present the first genome-scale model, iCJ415, for Streptococcus oralis SK141. We validated the model using gene essentiality and amino acid auxotrophy data from closely related species. iCJ415 has 71-76% accuracy in predicting gene essentiality and 85% accuracy in predicting amino acid auxotrophy. Further, the phenotype of S. oralis was tested using the Biolog Phenotype microarrays, giving iCJ415 a 82% accuracy in predicting carbon sources. iCJ415 can be used to explore the metabolic differences within the MGS, and to explore the complicated metabolic interactions between different species in the human oral cavity.
Highlights
The mitis group of streptococci (MGS) consists of 20 different species, all gram positive cocci arranged as pairs or in chains (Spellerberg and Brandt, 2008)
Most species in the MGS are considered commensal inhabitants of the oral cavity. These bacteria exist in a complex metabolic relationship with other oral streptococci, as well as other species residing in the oral cavity
The aim of this study was to construct a Genome-scale models (GEMs) of a S. oralis, the first GEM of a bacterium that is a major constituent of the human oral microbiome and an infective endocarditis (IE) causing bacterium
Summary
The mitis group of streptococci (MGS) consists of 20 different species, all gram positive cocci arranged as pairs or in chains (Spellerberg and Brandt, 2008). Most species in the MGS are considered commensal inhabitants of the oral cavity These bacteria exist in a complex metabolic relationship with other oral streptococci, as well as other species residing in the oral cavity. These interactions are thought to have an influence on the development of certain oral diseases in humans, e.g., caries (Kreth et al, 2009; Nascimento et al, 2009; Abranches et al, 2018). Of all the IE causing species in the MGS, Streptococcus oralis is the most common (Rasmussen et al, 2016)
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