Abstract
BackgroundSome lung diseases are cell type-specific. It is essential to study the cellular anatomy of the normal human lung for exploring the cellular origin of lung disease and the cell development trajectory.MethodsWe used the Seurat R package for data quality control. The principal component analysis (PCA) was used for linear dimensionality reduction. UMAP and tSNE were used for dimensionality reduction. Muonocle2 was used to extract lung epithelial cells to analyze the subtypes of epithelial cells further and to study the development of these cell subtypes.ResultsWe showed a total of 20154 high quality of cells from human normal lung tissue. They were initially divided into 17 clusters cells and then identified as seven types of cells, namely macrophages, monocytes, CD8 + T cells, epithelial cells, endothelial cells, adipocytes, and NK cells. 4240 epithelial cells were extracted for further analysis and they were divided into seven clusters. The seven cell clusters include alveolar cell, alveolar endothelial progenitor, ciliated cell, secretory cell, ionocyte cell, and a group of cells that are not clear at present. We show the development track of these subtypes of epithelial cells, in which we speculate that alveolar epithelial progenitor (AEP) is a kind of progenitor cells that can develop into alveolar cells, and find six essential genes that determine the cell fate, including AGER, RPL10, RPL9, RPS18, RPS27, and SFTPB.ConclusionWe provide a transcription map of human lung cells, especially the in-depth study on the development of epithelial cell subtypes, which will help us to study lung cell biology and lung diseases.
Highlights
In mammals, the lung is a highly branched network, in which the distal area of the bronchus tree transforms into dense alveolus vesicles during development
Researchers have been trying to restore tissue function after lung injury, which requires understanding how physiological tasks are distributed among cell types and how cell states change between homeostasis, damage/repair, and disease (Rock et al, 2010)
After Quality Control (QC), we furtherly analyzed a total of 20154 high-quality lung cells from four normal lung tissue
Summary
The lung is a highly branched network, in which the distal area of the bronchus tree transforms into dense alveolus vesicles during development. Developmental Trajectories of Lung Epithelium mucociliary clearance, and forming a barrier to inhaled particles and pathogens; (2) alveoli, the distal cystic structure where gas exchange takes place (Vieira Braga et al, 2019). The lung is a complex organ with long development time. It is controlled and coordinated by gene network and dynamic crosstalk of multiple cells, including lineage assignment, cell proliferation, differentiation, migration, morphogenesis, and damage repair. Researchers have been trying to restore tissue function after lung injury, which requires understanding how physiological tasks are distributed among cell types and how cell states change between homeostasis, damage/repair, and disease (Rock et al, 2010). It is essential to study the cellular anatomy of the normal human lung for exploring the cellular origin of lung disease and the cell development trajectory
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