Abstract
DEP1 and NRT1.1B genes for improving nitrogen utilization efficiency (NUE) have been widely used in japonica and indica rice (Oryza sativa L.) breeding, respectively. The aim of this study was to determine the effect of the non-allelic recombination of two genes on agronomic traits, grain yield and NUE under japonica and indica genetic backgrounds. One hundred and fifty-one recombinant inbred lines were derived from a cross between japonica variety SN265 with dep1/NRT1.1B alleles and indica variety R99 with DEP1/nrt1.1b alleles. Among these, we found four genotypes (DEP1/NRT1.1B, DEP1/nrt1.1b, dep1/NRT1.1B and dep1/nrt1.1b) under japonica and indica genetic backgrounds. Results showed a significant increase in nitrate reductase activity, N uptake and grain yield under low N growth condition, after the nrt1.1b allele was introduced into japonica lines with dep1. Whereas, the introduction of dep1 allele into indica lines with nrt1.1b increased glutamine synthetase activity, N transfer and grain yield under both low and high N growth conditions. Our results showed diverse physiological pathway between dep1 and nrt1.1b alleles in the N metabolism of rice, providing new strategies in flexible application of dep1 and nrt1.1b alleles for improvement of NUE in japonica and indica rice breeding.
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