Abstract
BackgroundInsulin-like growth factor I (IGF-I) is one important family of growth factors, which plays key role in intestinal growth, regeneration, and damage repair. However, the low natural abundance of IGF-I limits its research opportunities and practical application in the fields of medicine and animal husbandry. In this study, a tandem repeat strategy was used to express three copies of the same pIGF-I3 protein in L. lactis. The activity of recombinant pIGF-I3 (rpIGF-I3) was further examined by a mouse model of dextran sulfate sodium (DSS)–induced colitis. In addition, the potential of recombinant L. lactis expressing pIGF-I3 to reduce inflammatory disease was evaluated.ResultspIGF-I3 could be expressed in L. lactis by the detection of SDS–PAGE and Western blot. Experimental colitis was induced in BALB/c mice by administration of 5 % DSS in drinking water, and the clinical symptoms were observed in DSS-treated mice. Oral administration of recombinant L. lactis expressing pIGF-I3 improved the colonic architecture, and significantly reduced the increase of colonic damage score (P < 0.05). Furthermore, recombinant L. lactis expressing pIGF-I3 treatment significantly reduced serum DAO activity and colonic MPO level, and elevated colonic occludin level compared to the DSS group (P < 0.05).ConclusionsThe pIGF-I3 expressed in L. lactis has good biological activity, and oral administration of recombinant L. lactis expressing pIGF-I3 attenuated the symptoms and development of DSS-induced colitis in mice. These suggested that L. lactis could be a potential host bacterium for production and delivery of IGF-I against intestinal diseases.
Highlights
Insulin-like growth factor I (IGF-I) is one important family of growth factors, which plays key role in intestinal growth, regeneration, and damage repair
The rpIGF-I3 was further assayed by Western blot, and the result revealed that the corresponding immunoreactive band was present on the membrane from L. lactis NZ9000 (Fig. 1b)
These results indicated that L. lactis was able to express Three same genes of mature porcine IGF-I (pIGF-I3)
Summary
Insulin-like growth factor I (IGF-I) is one important family of growth factors, which plays key role in intestinal growth, regeneration, and damage repair. IGF-I is synthesized in the liver, produced locally in many tissues including the gastrointestinal tract, and regulated by growth hormone and nutrition [2, 3]. It exerts biological effect primarily through specific type I IGF receptor, which is distributed throughout the intestines of some mammalian, such as human, rat and pig [4, 5]. IGF-I can stimulate the proliferation and differentiation of many cell types, enhance DNA synthesis and protein content, inhibit cell apoptosis, and play an important role in the development and Studies have indicated that IGF-I stimulated intestinal growth and potentially regulated intestinal regeneration and repair. IGF-I has showed the potential to exert anti-inflammatory action by inhibiting pro-
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