Abstract
The application of whole cells as catalytic biofilms in microchannels has attracted increasing scientific interest. However, the excessive biomass formation and structure of biofilms in a reactor limits their use. A microchannel reactor with surface modification was used to colonize recombinant Escherichia coil BL21-pET28a-egfp rapidly and accelerated growth of biofilms in the microchannel. The segmented flow system of ‘air/culture medium containing nanomaterials’ was firstly used to modulate the biofilms formation of recombinant E. coil; the inhibitory effects of nanomaterials on biofilm formation were investigated. The results indicated that the segmental flow mode has a significant impact on the structure and development of biofilms. Using the channels modified by silane reagent, the culture time of biofilms (30 h) was reduced by 6 h compared to unmodified channels. With the addition of graphene sheets (10 mg/L) in Luria-Bertani (LB) medium, the graphene sheets possessed a minimum inhibition rate of 3.23% against recombinant E. coil. The biofilms cultivated by the LB medium with added graphene sheets were stably formed in 20 h; the formation time was 33.33% shorter than that by LB medium without graphene. The developed method provides an efficient and simple approach for rapid preparation of catalytic biofilms in microchannel reactors.
Highlights
Microorganisms mainly exist in nature in the form of biofilms [1]
Biofilms are mainly composed of surface-related microorganisms and flocs or aggregates formed by microorganisms, which can be adapted to various environments
In order to solve the problem of uncontrollable formation of biofilms, it is urgent to find a method for inhibiting the growth of biofilms
Summary
Microorganisms mainly exist in nature in the form of biofilms [1]. Biofilms are mainly composed of surface-related microorganisms and flocs or aggregates formed by microorganisms, which can be adapted to various environments. Excellent works have been done for biofilm colonization and the growth of catalytic biofilms could be controlled by aqueous-air segmented flow in continuous microreactors [12] With this method, the epoxidation of styrene to (S)-styrene oxide As a new type of highly efficient adsorption carrier, have the advantages of larger specific surface area, higher loading capacity, ordered nanopore structure, excellent chemical stability, and biocompatibility [17]. The impacts of surface modification and nanomaterials on the formation and structure of biofilms in a microreactor were investigated This method is highly promising in the application of catalytic biofilms of recombination strains expressing enzymes in microreactors
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