Abstract
The estimation of alanine, valine and leucine can be carried out by transforming each of these amino acids into the corresponding aldehyde by the action of sodium hypochlorite. The aldehyde so formed is removed by steam or a stream of air and collected in bisulphite. The estimation of alanine is thus reduced to the estimation of acetaldehyde; and it is specific if care is taken to remove aspartic acid from the reaction mixture, and if the aldehyde is estimated either by nitroprusside and piperazine or by p-hydroxydiphenyl. The separation of the aldehydes corresponding to valine and leucine is carried out after removal by fractional distillation, in the presence of carbon tetrachloride, in a sufficiently powerful column. Under these conditions it is possible to estimate in any mixture of amino acids, and separately, alanine, valine and the leucine group. The accuracy of the results is of the order of 5%.
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