Abstract
Increasing concerns about nosocomial infection, food and environmental safety have prompted the development of rapid, accurate, specific and ultrasensitive methods for the early detection of critical pathogens. Pseudomonas aeruginosa is one of the most common pathogens that cause infection. It is ubiquitous in nature, being found in water, soil, and food, and poses a great threat to public health. The conventional detection technologies are either time consuming or readily produce false positive/negative results, which makes them unsuitable for early diagnosis and spot detection of P. aeruginosa. To circumvent these drawbacks, many efforts have been made to develop biosensors using aptamers as bio-recognition elements. Various aptamer-based biosensors for clinical diagnostics, food, and environmental monitoring of P. aeruginosa have been developed in recent years. In this review, we focus on the latest advances in aptamer-based biosensors for detection of P. aeruginosa. Representative biosensors are outlined according to their sensing mechanisms, which include optical, electrochemical and other signal transduction methods. Possible future trends in aptamer biosensors for pathogen detection are also outlined.
Highlights
Pseudomonas aeruginosa, an opportunistic Gram-negative bacterium, is one of the most intractable multidrug-resistant bacteria causing severe nosocomial infections and poses an increasing threat to human health (Curran et al, 2018; Horcajada et al, 2019; Jean et al, 2020)
Immunological tests are commonly used for clinical diagnoses. They are dependent on antibodies, which are sensitive to temperature and pH changes, and prone to false negatives/positives (Mauch and Levy, 2014)
Bacterial enrichment is needed before analysis, resulting in a long turnaround time (Perry, 2017)
Summary
Pseudomonas aeruginosa, an opportunistic Gram-negative bacterium, is one of the most intractable multidrug-resistant bacteria causing severe nosocomial infections and poses an increasing threat to human health (Curran et al, 2018; Horcajada et al, 2019; Jean et al, 2020). In 2017, P. aeruginosa was listed as a critical pathogen by the World Health Organization. It is widely distributed in soil, water, air, animals and humans. P. aeruginosa tends to form biofilm, which is difficult to eradicate and has 10- to 1,000-fold higher resistance to antibiotic concentrations compared with equivalent floating bacteria
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