Abstract
Most cytotoxic agents have limited efficacy for solid cancers. Cell-cycle phase analysis at the single-cell level in solid tumors has shown that the majority of cancer cells in tumors is not cycling and is therefore resistant to cytotoxic chemotherapy. Intravital cell-cycle imaging within tumors demonstrated the cell-cycle position and distribution of cancer cells within a tumor, and cell-cycle dynamics during chemotherapy. Understanding cell-cycle dynamics within tumors should provide important insights into novel treatment strategies.
Highlights
We focus on intravital imaging with the fluorescence ubiquitination-based cell cycle indicator (FUCCI) imaging of individual cells in tumors
Cells 2018, 7, 168 we focus on intravital imaging with the fluorescence ubiquitination-based2 of cell cycle indicator (FUCCI) imaging of individual cells in tumors
These results suggested that FUCCI-red quiescent cancer cells are resistant to irradiation and play a key role in metastasis
Summary
Intravital fluorescence imaging enables visualization of live cancer cell dynamics including proliferation, invasion, and metastasis [1,2,3,4]. We focus on intravital imaging with the fluorescence ubiquitination-based cell cycle indicator (FUCCI) imaging of individual cells in tumors. FUCCI imaging enabled visualization of cell-cycle dynamics of phase, and yellow nuclei in FUCCI-expressing cells (FUCCI-yellow) indicate the early S phase FUCCI for intravital real-time in vivo imaging of cancer cells at the single cell level met these criteria. Imaging of FUCCI-expressing gastric-cancer cells growing in the liver using a skin-flap window. CLSM imaging of FUCCI-expressing gastric cancer cells growing in the liver using an abdominal skin-flap window. Longitudinal Intravital Imaging of an Orthotopic Metastatic Liver Tumor Model with FUCCI
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