Abstract
Reagentless amperometric hydrogen peroxide and L-lactate electrodes based on bienzyme modified carbon pastes were tested and compared. The behaviour of 7 peroxidases and two lactate oxidases immobilized in carbon pastes were investigated amperometrically under flow injection conditions. By the combination of recombinant lactate oxidase from Aerococcus viridans (RLOD) and lactate oxidase from Pedicoccus sp. with (LOD) horseradish peroxidase, peroxidase form Arthromyces ramosus, microperoxidases MP-8 and MP-11 sensitive carbon paste electrodes were prepared to detect H2O2 and L-lactate. The selectivity with respect to several redox active substances and substrates of the peroxidases is slightly influenced by the selection of the heme containing peroxidase. The operational stability of both the hydrogen peroxide and the L-lactate sensors could be improved by electrochemical deposition of o-phenylenediamine onto bienzyme modified carbon paste electrodes. Despite microperoxidases have a considerably lower electro-catalytical efficiency per molecule, especially MP-8 can be used to modify carbon paste electrodes for sensitive H2O2 detection. Both MP-8 and MP-11 can be coimmobilized with LOD and RLOD to detect L-lactate.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
