SummaryHigh-throughput image-based technologies are now widely used in the rapidly developing field of digital phenomics and are generating ever-increasing amounts and diversity of data. Artificial intelligence (AI) is becoming a game changer in turning the vast seas of data into valuable predictions and insights. However, this requires specialized programming skills and an in-depth understanding of machine learning, deep learning, and ensemble learning algorithms. Here, we attempt to methodically review the usage of different tools, technologies, and services available to the phenomics data community and show how they can be applied to selected problems in explainable AI-based image analysis. This tutorial provides practical and useful resources for novices and experts to harness the potential of the phenomic data in explainable AI-led breeding programs.

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