Abstract
Influence of polyethylene glycol (PEG) mediated osmotic stress on reactive oxygen species (ROS) scavenging machinery of Chinese potato (Solenostemon rotundifolius (Poir.) J. K. Morton) was investigated. Five genotypes of Chinese potato were raised in Murashige and Skoog (MS) basal medium containing 6–benzylaminopurine (BAP, 1 mg L–1) along with various concentrations of PEG–6000 mediated stress conditions (0, –0.2 and –0.5 MPa) and evaluated for osmotic stress tolerance in vitro. The medium containing PEG–6000 had a detrimental effect on plantlet growth and development while compared with the control. Accumulation of H2O2 was lower in Sreedhara and Subala and higher in Nidhi under PEG stress, which was evident by in situ detection in leaves. Lipid peroxidation product such as malondialdehyde (MDA) content was increased due to PEG stress which was more in susceptible genotype than that in tolerant ones. An enhanced ROS–scavenging antioxidant enzyme was observed under stress with respect to the control. The enzymes of ascorbate–glutathione cycle showed an important role in scavenging ROS. The imposition of PEG stress also increased the non–enzymatic antioxidants viz., the ascorbate and reduced glutathione content which was prominent in tolerant genotypes in comparison to susceptible. The present study indicated that, Sreedhara and Subala showed more tolerance to osmotic stress with better ROS scavenging machineries which would be the lines of interest for augmenting future breeding strategies in this climate resilient minor tuber crop.
Highlights
Influence of polyethylene glycol (PEG) mediated osmotic stress on reactive oxygen species (ROS) scavenging machinery of Chinese potato (Solenostemon rotundifolius (Poir.) J
Under PEG stress condition, Subala and Sreedhara maintained the growth by registering up to 20% decrease in shoot proliferation and rooting, which reflects its inherent tolerance towards osmotic stress
Our results showed that ascorbate–glutathione cycle (ASA–GSH) played an important role on scavenging the over production of H2O2 due to osmotic stress in Chinese potato genotypes
Summary
Influence of polyethylene glycol (PEG) mediated osmotic stress on reactive oxygen species (ROS) scavenging machinery of Chinese potato (Solenostemon rotundifolius (Poir.) J. To elucidate the plant response to osmotic stress, in vitro cultures are preferred as it minimizes nutrient and environmental variations under control conditions[4]. In this investigation, we have used polyethylene glycol (PEG–6000) to induce osmotic stress conditions in vitro. A major H2O2 detoxifying system operating in plants is associated with ‘ascorbate–glutathione cycle’ (ASA–GSH) consisting of four enzymatic antioxidative machineries In this cycle, ascorbate peroxidase (APX) efficiently manages H2O2 during stress than CAT. The peroxidation of membrane lipids and its role in osmotic stress tolerance in Chinese potato is still unexplored
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