Abstract
Charge recombination in the primary radical pair P +H − of the purple photosynthetic bacterium Rp. viridis with pre-reduced secondary acceptor Q A, has been studied by time-resolved photovoltage measurements and transient absorption spectroscopy with a time resolution of 1 ns. The lifetime of P +H − was found to be 2.4–3 ns in intact membranes and ∼5 ns in isolated reaction centers, in contrast to a lifetime of ∼15 ns which has been widely accepted in the literature for reaction centers. The origin of the difference between lifetimes of P +H − in membranes and isolated reaction centers is discussed.
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