Abstract
Podophyllum hexandrum Royle is an important high-altitude plant of Himalayas with immense medicinal value. Earlier, it was reported that the cell wall hydrolases were up accumulated during radicle protrusion step of Podophyllum seed germination. In the present study, Podophyllum seed Germination protein interaction Network (PGN) was constructed by using the differentially accumulated protein (DAP) data set of Podophyllum during the radicle protrusion step of seed germination, with reference to Arabidopsis protein–protein interaction network (AtPIN). The developed PGN is comprised of a giant cluster with 1028 proteins having 10,519 interactions and a few small clusters with relevant gene ontological signatures. In this analysis, a germination pathway related cluster which is also central to the topology and information dynamics of PGN was obtained with a set of 60 key proteins. Among these, eight proteins which are known to be involved in signaling, metabolism, protein modification, cell wall modification, and cell cycle regulation processes were found commonly highlighted in both the proteomic and interactome analysis. The systems-level analysis of PGN identified the key proteins involved in radicle protrusion step of seed germination in Podophyllum.
Highlights
Podophyllum hexandrum Royle (= SinoPodophyllum hexandrum) known as “Indian mayapple,” is an important Himalayan medicinal herb exploited for its etoposides which are potential anticancer compounds
differentially accumulated protein (DAP) during the radicle protrusion step of seed germination of Podophyllum i.e., Podophyllum Germination Proteins (PGPs) reported by Dogra et al (2013), are used as the data set for the construction of Podophyllum germination protein interaction network i.e., Podophyllum Germination Network (PGN)
Orthologs is composed of PGPs in Arabidopsis thaliana were considered as functional equivalent representatives and were mapped onto the Arabidopsis protein–protein interaction network (AtPIN)
Summary
Podophyllum hexandrum Royle (= SinoPodophyllum hexandrum) known as “Indian mayapple,” is an important Himalayan medicinal herb exploited for its etoposides which are potential anticancer compounds. In order for a seed to complete germination, the growth potential of the radicle must overcome the tissue resistance of the seed covering layers (Leubner-Metzger et al, 1995; Bewley, 1997; Rosental et al, 2014). In our earlier studies on Podophyllum hexandrum, it was found that presence of thick-walled cells at the micropylar endosperm, delays the radicle protrusion during seed germination (Sreenivasulu et al, 2009). We confirmed that these differentially accumulated proteins (DAPs) are critical for germination processes in Podophyllum (Dogra et al, 2013; Dogra and Sreenivasulu, 2015)
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