Ratlarda böbrek iskemi-reperfüzyon hasarında proflaktik kalsiyum dobesilatın etkinliği

Abstract

Aim: In this study, the objective was to investigate the protective effect of calcium dobesilate, which has antioxidant and anti-inflammatory properties, on the experimental renal ischemia-reperfusion injury (IRI). Methods: Twenty-four male Wistar-Albino rats were divided into three groups: Sham group (Group 1), ischemia-reperfusion group (Group 2), and treatment group (Group 3). Before the ischemia-reperfusion procedure, rats in Group 3 received calcium dobesilate through gavage (100mg/kg/day) for 10 days. Groups other than the sham group underwent ischemia for 45 minutes and reperfusion for 24 hours. Plasma urea and creatinine levels, erythrocyte superoxide dismutase and glutathione peroxidase enzyme activity levels were measured. In addition, histopathological changes that may be related to ischemia-reperfusion injury in the renal tissue, were investigated.Results: The median glutathione peroxidase and superoxide dismutase enzyme levels were higher in Group 2 compared to Groups 1 and 3. However, the differences were not statistically significant. The creatine levels were statistically lower in Group 3 compared to Group 1 and Group 2. The median urea levels were lower in Group 3 than in Group 1 and Group 2, but the differences were not statistically significant. The histopathological examination showed that parameters such as cellular necrosis, flattened tubular epithelial cells, cytoplasmic vacuolization, tubular lumen obstruction, and chronic inflammation, which are indicators of the ischemia-reperfusion injury, were statistically less common in the treatment group compared to the control group. Conclusion: Our study demonstrated that prophylactic calcium dobesilate had a protective effect on ischemia-reperfusion injury.