Abstract
Detailed studies of the pH stability range of infectivity of plant viruses, summarized recently by Stanley,1 show that for each virus studied there is a well defined region where infectivity is relatively stable. On both sides of this region infectivity is lost in a regular way so that the curve plotted to relate infectivity with pH of the medium is smooth and trapezoidal. Certain of the animal viruses2, 3 appear to behave in a like manner. It was entirely unexpected, then, when conditions were encountered under which the virus of equine encephalomyelitis (Eastern Strain) seemed to behave in a different way.The experiments were made on virus propagated in chick embryos.4 Virus-infected embryos were “harvested” when moribund and ground with sand in hormone broth to a 10% suspension. Centrifuged free of sand and gross tissue particles, the whole extract was mixed with composite buffer solution (0.05M) of various pH's.2, 3 Mixtures were maintained between 0°C and 5°C, and after various intervals, usually 1 h...
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