Abstract
Hydrogen peroxide (H2O2) is usually used as a fungicide in food, it is carcinogenic, accelerates aging or inducing toxic effects such as cardiovascular disease. Herein, to meet the demand for effective and fast detection of H2O2 in food, a novel non-enzymatic electrochemiluminescence (ECL) sensor based on single-stranded DNA (ssDNA)/g-C3N4 nanosheets (NS) was established. The ssDNA/g-C3N4 NS hybrid was prepared by simple mixing g-C3N4 NS and ssDNA solution together. The prepared ssDNA/g-C3N4 NS exhibited improved peroxidase-like activity and was modified on a glassy carbon electrode to catalyze the ECL reaction of luminol-H2O2 to amplify the luminescence signal. Under the optimized conditions, the proposed sensor exhibits high sensitivity with a limit of detection (LOD) as low as 33 aM H2O2, which is much lower than the vast majority of reported methods. This method enables the reliable responding to H2O2 from the milk samples within 1 min.
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