Abstract
A very rapid method for isolating bacterial photosynthetic reaction centers (RCs) of high purity and yield is described. A poly-histidine tag is engineered at the C-terminus of the RC M-subunit, allowing recovery of pure RCs in less than 4 h with a commercially available Ni 2+ resin. The phenotype of the protein including absorption spectra and electron transfer kinetics are virtually identical to wild-type RCs. This method should facilitate studies of RCs by dramatically decreasing sample preparation time and eliminating the use of expensive equipment.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Bioenergetics
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