Abstract
Screening for colonization with methicillin resistant Staphylococcus aureas (MRSA) is a key aspect of infection control to limit the nosocomial spread of this organism. Current methods for the detection of MRSA in clinical microbiology laboratories using conventional methods is time consuming. In this research we are trying to evaluate the use of real time PCR for the detection of MRSA. The PCR assay was evaluated in clinical isolates of MRSA (n = 45) and methicillin susceptible Staphylococcus aureas MSSA (n = 10). The diagnostic values of the assay showed high sensitivity and specificity. This real-time PCR assay proved to be a fast, sensitive and specific tool for MRSA detection in a routine microbiological laboratory. Real-time PCR now is available in all laboratories so its use in identification of MRSA will help in shortening the period for MRSA identification and will help in the success of infection control programs in hospitals.
Highlights
Staphylococci are ubiquitous colonizers of human or animal skin and mucous membranes causing a variety of syndromes
The current study aimed to evaluate the use of real time PCR in detection of methicillin resistant Staphylococcus aureus (MRSA) and to trace the dynamics of genotypic changes and the shifts in the levels of susceptibility to antibiotics of methicillin resistant Staphylococcus aureas (MRSA) isolated from patients in King Abdel Aziz Hospital, Al-Taif, Saudi Arabia during the period from Jan. to Jun. 2012
Upon compareing our results of antibiotic susceptibility with other results inside Saudi Arabia, we found that all results (Table 3) in consistent with the results of Moussa and Hessan [15] except for ciprofloxacin in which our results showed only 30% of MRSA isolates were susceptible in comparison to 60% in their results
Summary
Staphylococci are ubiquitous colonizers of human or animal skin and mucous membranes causing a variety of syndromes. Staphylococcus aureus can cause nosocomial and community-acquired infections ranging from mild conditions, such as skin and soft tissue infections, to severe, life-threatening sepsis [1]. Strains of methicillinresistant S. aureus (MRSA) were first detected in the early 1960s, shortly after methicillin came into clinical use [2]. An increase in the number of Methicillin resistant Staphylococcus aureus (MRSA) cases has been widespread globally among healthy community [3]. A core function of all bacteriology laboratories is to determine the antibiotic susceptibilities of bacterial isolates and to detect the resistance phenotypes conferred by these resistant genes. If a panel of agents in a particular antibiotic class is tested, the interpretative reading of the resulting patterns of resistance or susceptibility often suggests the underlying resistance mechanisms, predicts second-line drugs to be tested and pro-
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