Abstract

Antioxidants belong to one of the most important group of compounds presented in herbs, nutraceuticals and functional food, which prevent oxidative stress avoiding cell damage. Hence, antioxidants have a high demanding and also their natural sources. A new procedure has been used to separate and quantify the free radical-scavenging activity of individual compounds from 44 samples of Calendula officinalis, 18 samples of Thymus vulgaris and 12 samples of Rosmarinus officinalis, based on the combination of HPTLC with a diode array detector (DAD) and postchromatographic DPPH● radical derivatization.

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