Abstract
A rapid and specific method for the determination of bleomycin A2 is described. A 50-μl aliquot of 20% trichloroacetic acid was added to 200μl of plasma. The sample was vortexed and centrifuged, and 50μl of the clear supernate was injected into a liquid chromatograph equipped with a microparticulate reversed-phase column and a fixed wavelength detector. Elution was carried out using methanol–acetonitrile–0.0085M heptanesulfonic acid–acetic acid. A linear calibration curve was found in the 0.05–5–μg/ml range with an estimated precision of±6% (CV). Preliminary pharmacokinetic data in the rabbit also are reported.
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