Abstract
Thiabendazole is widely used in sclerotium blight, downy mildew and black rot prevention and treatment in rape. Accurate monitoring of thiabendazole pesticides in plants will prevent potential adverse effects to the Environment and human health. Surface Enhanced Raman Spectroscopy (SERS) is a highly sensitive fingerprint with the advantages of simple operation, convenient portability and high detection efficiency. In this paper, a rapid determination method of thiabendazole pesticides in rape was conducted combining SERS with chemometric methods. The original SERS were pretreated and the partial least squares (PLS) was applied to establish the prediction model between SERS and thiabendazole pesticides in rape. As a result, the SERS enhancing effect based on silver Nano-substrate was better than that of gold Nano-substrate, where the detection limit of thiabendazole pesticides in rape could reach 0.1 mg/L. Moreover, 782, 1007 and 1576 cm−1 could be determined as thiabendazole pesticides Raman characteristic peaks in rape. The prediction effect of thiabendazole pesticides in rape was the best ( = 0.94, RMSEP = 3.17 mg/L) after the original spectra preprocessed with 1st-Derivative, and the linear relevance between thiabendazole pesticides concentration and Raman peak intensity at 782 cm−1 was the highest (R2 = 0.91). Furthermore, five rape samples with unknown thiabendazole pesticides concentration were used to verify the accuracy and reliability of this method. It was showed that prediction relative standard deviation was 0.70–9.85%, recovery rate was 94.71–118.92% and t value was −1.489. In conclusion, the thiabendazole pesticides in rape could be rapidly and accurately detected by SERS, which was beneficial to provide a rapid, accurate and reliable scheme for the detection of pesticides residues in agriculture products.
Highlights
Thiabendazole, a benzimidazole derivative which belongs to the absorption of a broad-spectrum fungicide, has been widely used in rape sclerotium blight, downy mildew and black rot disease prevention
The results suggested that the predicted value was basically same as the measured value of Ultra high performance liquid chromatography (UHPLC) method, which indicated that the rapid detection of thiabendazole pesticide in rape by SERS was feasible
(2) The prediction results (R2p = 0.94, RMSEP = 3.17 mg/L) was the best combined with 1st-Der preprocessing and partial least squares (PLS) modeling
Summary
Thiabendazole, a benzimidazole derivative which belongs to the absorption of a broad-spectrum fungicide, has been widely used in rape sclerotium blight, downy mildew and black rot disease prevention. The long-term exposure or misuse of thiabendazole pesticides could lead to a variety diseases such as cancer, blood diseases and immune system disorders, which seriously affects the physical and mental health [1]. In accordance with the provisions of the maximal residue of pesticides in food in China (GB 2763-2014), the thiabendazole pesticides in rape cannot exceed 1 mg/kg.
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