Abstract
<p>The aim of the present work was the development of a novel amperometric biosensor for rapid detection of sucrose in fruit juices samples. Two enzymes, invertase and fructose dehydrogenase (FDH), were immobilized onto a single-walled carbon nanotube paste (SWCNTP) electrode by wiring with a highly original osmium-polymer hydrogel. A second biosensor, for fructose only, was constructed containing inactive invertase and used for signal subtraction.</p> <p>The biosensor exhibits a detection limit for sucrose of 2 mM, linearity up to 5 mM, good sensitivity of 1.98 mA cm<sup>-2</sup> mM, good reproducibility (RSD = 2.5%), fast response time (8s) and a stability of 4 months if kept under wet conditions at 4 °C.</p> <p>The biosensor was successively tested for specific detection of sucrose and fructose in several commercial fruit juice samples and the results were compared with those obtained with a commercial spectrophotometric enzymatic kit.</p>
Highlights
Sucrose is an important monosaccharide contained in many foodstaffs and sweet drinks
We propose a more convenient method which used only two enzymes, invertase and fructose dehydrogenase, immobilized onto a single-walled carbon nanotube paste (SWCNTP) electrode properly modified with a highly original osmium-polymer hydrogel
The operating mechanism of the sucrose biosensor is represented by the following reactions: Invertase sucrose + H2O -D-glucose + D-fructose
Summary
Sucrose is an important monosaccharide contained in many foodstaffs and sweet drinks. Various conventional analytical methods are largely reported in literature for the determination of sucrose, such as gas-chromatography, UV-spectrophotometry, titration and electrophoresis (AOAC, 1995; Beutler, 1984). All these methods require expensive instrumentation, long time of analysis, well trained operators and sometimes complicated sample pretreatments. It is of extreme importance to develop a fast, selective and affordable method usable by food industries. Biosensors can represent a unique alternative: they are low cost devices with very good sensitivity and selectivity which can be used “in situ” without long or difficult sample pretreatments (Tran & Cahn, 1993; Eggins, 2002; Wagner & Guilbault, 1994)
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