Abstract

To determine the content of puerarin in Xiaoke pill powder rapidly by near-infrared spectroscopy. Near-infrared diffuse reflectance spectroscopy technology was used to collect NIR spectra of Xiaoke pills powder. With HPLC analysis values as reference, the fast determination method of puerarin was established with partial least squares (PLS). The R2, RMSECV and RPD of the calibration model for puerarin were 0.980 1, 0.131 and 7.09. The average relative deviation of the predication set was 3.2%. The method is accurate, fast and simple, which could be generalized to the on-line quality control of Xiaoke pill powder production.

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