Abstract
A surface-enhanced Raman spectroscopy (SERS) method for the determination of isepamicin (ISE) using silver nanoparticles (AgNPs) protected by bovine serum albumin (BSA) and modified by α-Fe2O3 as an efficient substrate was established. The synthesized substrate was characterized and verified by transmission electron microscopy (TEM), ultraviolet visible spectroscopy (UV–vis), and fourier transform infrared spectroscopy (FT-IR). The conditions affecting the Raman signal of ISE were optimized by single factor and response surface experiments. Under optimized conditions, a standard curve ISERS = 43.08c + 63598.69 (c: nmol/L) with a linear relationship (r = 0.9976) was established between the SERS intensity and ISE concentration in the range of 20.00 – 2000.00 nmol/L. The limit of detection (LOD) for ISE was 16.58 nmol/L (S/N = 3). The recovery of ISE in the samples was 96.29 % – 104.12 %, with relative standard deviation (RSD) was 1.53 % – 3.43 % (n = 5). The SERS method was reliable and satisfactory for the quantitative analysis of ISE at low concentration.
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