Abstract
Glucose and sucrose are simultaneously determined by the use of an enzyme sensor system consisting of a glucose-sensing electrode based on a lipid-modified glucose oxidase and a measuring cell that contains an invertase/mutarotase-coimmobilized layer. From the current response of the enzyme electrode after the addition of a glucose/sucrose mixture, the concentrations of the two kinds of sugars can be separately determined: the concentration of glucose (0·2 μM–3 mM) is determined from the steady-state current increase obtained from 2 to 6 s after the addition of the mixture, and that of sucrose (10 μM–6 mM), from the rate of current increase from 8 to 20 s after the addition. The relative standard deviations are 1·7% for glucose and 3·1% for sucrose ( n=10). The system can be applied to the rapid determination of glucose and sucrose in food samples.
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