Abstract
In this work, a quartz crystal microbalance (QCM) sensor has been fabricated using immunoassay for sensitive determination of Bifidobacterium bifidum. Au nanoparticle has been used for amplifying sandwich assays. The proposed immunosensor exhibited a linear detection range between 103 and 105 CFU/mL with a limit of detection of 2.1 × 102 CFU/mL. The proposed immunosensor exhibited good selectivity for B. bifidum sensing with low cross reactivity for other foodborne pathogens such as Lactobacillus acidophilus, Listeria monocytogenes, and Escherichia coli. In addition, the proposed immunosensor has been successfully used for B. bifidum detection in feces samples and food samples. The frequency decreases of 12, 17, and 10 Hz were observed from the milk samples consisting of the mixtures of L. acidophilus, L. monocytogenes, and E. coli. The frequency decreases of 8, 15, and 7 Hz were observed from the feces samples consisting of the mixtures of L. acidophilus, L. monocytogenes, and E. coli.
Highlights
IntroductionThe intestinal microorganisms in the human body form a relatively balanced state
Under normal circumstances, the intestinal microorganisms in the human body form a relatively balanced state
In order to further enhance the sensitivity of the quartz crystal microbalance (QCM) immunosensor, Au nanoparticles were introduced for forming the antibody-AuNPs conjugation (Zheng et al, 2015; Wang L. et al, 2017; Fu et al, 2020; Xu et al, 2020)
Summary
The intestinal microorganisms in the human body form a relatively balanced state. Once the balance is damaged, it will lead to the imbalance of intestinal flora (Horie et al, 2017; Xue et al, 2018) Some intestinal microorganisms, such as Clostridium perfringens, overproduce in the intestinal tract and produce harmful substances such as ammonia, amines, hydrogen sulfide, manure, indole, nitrite, and bacterial toxins, which will further affect the health of the body (Ashida et al, 2018; Duranti et al, 2019). B. bifidum can inhibit the growth of harmful bacteria, resist the infection of pathogenic bacteria, and synthesize vitamins needed by human body.
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