Rapid and specific ria of serum estrone sulfate with selective solid phase extraction

Abstract

The methods commonly used to evaluate conjugated steroids require hydrolysis and Chromatographie purification. To avoid these steps, a simple method involving selective solid phase extraction and RIA using a highly specific antiserum for estrone sulfate (E 1 S) has been evolved. A Bond-Elut C 2 cartridge was used for solid phase extraction of estrone (E 1) and E, S; recoveries were 80 and 90% respectively. The intra- and inter assay precision of the assay at 3 serum levels, were 6.5, 10.4 and 4.4 and 12.7, 13.9 and 7.4% respectively. Accuracy, tested by linearity and recovery tests, was acceptable. A good correlation exists between a conventional enzymatic method and the proposed method. The latter is less time consuming and more reliable, thus providing a rapid assay to evaluate E 1 and E 1 S in the same serum sample.