Abstract
Difenoconazole is triazole fungicide, widely used to prevent the growth and reproduction of fungi and to control fungal diseases in fruits and vegetables. In this work, we prepared a hapten of difenoconazole and produced a highly-sensitive anti difenoconazole-monoclonal antibody (mAb) with an IC50 of 0.64 ng/mL. Using the mAb, an immunochromatographic assay (ICA) was developed to analyze difenoconazole residues in pear and cucumber samples. The ICA exhibited LOD values of 2.60 and 2.30 ng/g for difenoconazole in pear and cucumber samples, respectively. The ICA showed average recoveries of difenoconazole ranging from 94.3% ± 3.6%–105.4% ± 1.6%, and CVs of 1.5–4.2% in cucumber and pear samples. When used to measure difenoconazole in samples, the ICA results were compatible with those detected by LC/MSMS. The results of this study support the idea that an ICA is a practical and effective tool for high-throughput and rapid analysis of difenoconazole residues in vegetables and fruits.
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