Abstract
Molecularly imprinted polymers (MIPs) specifically targeting pentachloronitrobenzene (PCNB) and containing silver nanoparticles have been prepared by free radical polymerization reaction using methyl methacrylate (MMA) as a functional monomer, PCNB as a template molecule, 1,4-butanedioldimethacrylate as a cross linker, lauroyl peroxide (LPO) as an initiator, and the silver nanoparticles with the best surface-enhanced Raman scattering (SERS) effect as SERS enhancement materials. Our results indicated that MIPs specifically recognize PCNB from complex matrices. The intensity of the PCNB characteristic peak was proportional to the concentration, with a linear range of 0.005 to 0.15 μg/mL and a limit of detection of 5.0 ng/mL. The recovery rates and relative standard deviation for the detection of PCNB spiked in the rice samples were from 94.4% to 103.3% and from 4.6% to 7.4%, respectively. The experimental results are consistent with those by the GC-MS method, indicating that the rapid detection of PCNB in food matrices by SERS-MIPs is reliable. In view of the insolubility of PCNB in water, oil-soluble silver nanoparticles were synthesized which can be expanded to detect oil-soluble toxic substances. For the first time, the proposed method provides a point-of-care and cost-effective tool for rapidly detecting PCNB in food matrices with high sensitivity and selectivity by employing SERS-MIPs method.
Highlights
The prepared Molecularly imprinted polymers (MIPs), which was embedded with oil-soluble silver nanoparticles as surface-enhanced Raman scattering (SERS) substrate, can recognize PCNB, providing a sensitive, selective, onsite, and rapid detection method for PCNB residue in food by SERS-MIPs
By using oleamine and oleic acid as protective agents, the silver nanoparticles were soluble in organic phase so that they could contact with PCNB molecules
The linear range was 0.005 to 0.15 μg/mL and the limitofofquantification detection (LOD) was 0.005 μg/mL for the detection of PCNB spiked in water, and the corresponding recoveries ranged from 95.0% to
Summary
Pesticides play an important role in preventing crop diseases and pests and in maintaining agricultural production. The main mechanism of action of PCNB is to prevent and control plant pathogens by interfering with cell mitosis and inhibiting spore formation. It mainly uses seed coating or seed dressing methods to control cotton seedling diseases, blight disease, wheat, sorghum smut, gold worm, or soil treatment methods to control eggplant blight, cataplash disease, and watermelon blight control by irrigation. Due to its high efficiency, low cost, and long-lasting effects, it has been widely used in the control of many kinds of seed diseases and pests [1]
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